Mutation yedK777(del)::kan
- Name: yedK777(del)::kan
- Type: Insertion
- Mutation of Gene: yedK
- Approx. Map Location: 43.28
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ACCCGCCCCCACTCCTATTCTTTTCAGTATGATCTCAGGAGGTTTCTATGATTCCGGGGATCCGTCGACC and GTATTGTTGATCAATAATCTCCAGGCTTATCAAACAGGTTGAATTAACTCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1916-5 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyedK777::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514