Mutation yggU727(del)::kan
- Name: yggU727(del)::kan
- Type: Insertion
- Mutation of Gene: yggU
- Approx. Map Location: 66.69
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGATTCCGGGGATCCGTCGACC and TTGCGAGGACAACTTTTTGCATAGGATACCTAATTAATTAACGCCGCGATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5479-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyggU727::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514