Mutation yhhJ740(del)::kan
- Name: yhhJ740(del)::kan
- Type: Insertion
- Mutation of Gene: yhhJ
- Approx. Map Location: 78.10
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CTGGTGATGGGCTTAAGTATCCTGCTGCTGAAAAAACAGGAGGGATGATGATTCCGGGGATCCGTCGACC and CTATAAGCTTACGCATCGGGCAATTGTATTTACGCCATTGTCCCAATCGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5677-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔyhhJ740::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514