Mutation yicC750(del)::kan
- Name: yicC750(del)::kan
- Type: Insertion
- Mutation of Gene: yicC
- Approx. Map Location: 82.22
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GGCGGAAAAGTAAGAATTGGCGTTACGAGTTATTCGATGTTCTGAATCTGATTCCGGGGATCCGTCGACC and CGATGGCTATAATCCTTCCACCTCTCCTTTTATAAACAGGAACGTCTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW3619-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔyicC750::kan, Δ(rhaD-rhaB)568, hsdR514