Strain - WTZ18004

Strain - WTZ18004Strain WTZ18004

Strain WTZ18004

Designation	Source	Preference
WTZ18004	Wertz J.E.	1

8 Mutations:

Mutation	Location	Certainty	Display
DE(araD-araB)567	1.40	1	Δ(araD-araB)567
lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
LAM-	17.40	1	λ^-
relA882(del)::FRT	62.71	1	ΔrelA882::FRT
rph-1	82.20	1	rph-1
bipA733(del)::kan	87.43	1	ΔbipA733::kan
DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
hsdR514	98.74	1	hsdR514

Comments:
- This is a double knockout constructed from Keio strains by the CGSC
- This strain should be grown at 37 deg. C and tends to grow slowly. Growth at lower temperatures will tend to select for rluC mutations that suppress the cold sensitive phenotype of the bipA deletion.
References:
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
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