Strain - BW22245/pWM71

Strain - BW22245/pWM71Strain BW22245/pWM71

Strain BW22245/pWM71

CGSC#: 8214
Strain Designation
Designation Source Preference
BW22245/pWM71 Wanner B.L. 1
Sex(Hfr,F+,F-,or F'): F-

Designation	Source	Preference
BW22245/pWM71	Wanner B.L.	1

7 Mutations:

Mutation	Location	Certainty	Display
DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
phoA532(del)	8.64	1	ΔphoA532
uidA4(del)::pir-116	36.48	1	ΔuidA4::pir-116
recA1	60.80	1	recA1
rpoS396(Am)	61.75	0	rpoS396(Am)
rph-1	82.20	1	rph-1
DE(phnP-phnD)3330( phnC?)	92.93	1	Δ(phnP-phnD)3330( phnC?)

Comments:
- pWM71 is like pAH34 except for PstI site in bla. pAH34 host strain is pir⁺ and F^-.
Episome/Plasmid: F128-Tn10-11
Plasmid Markers/Mutations:
F::Tn10-11
lacIp-4000(lacIQ)
lacZ58(del)(M15)
proA
proB
Tn10-11
lac
Episome/Plasmid: pWM71
Plasmid Markers/Mutations:
bla(ApR)
lacZ alpha
MCS
ori f1,+
oriR6Kgamma
oriT RP4
proA
proB
Tn10-11
References:
- Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
Printable Strain Report