Strain - JM109/pWM5

Strain - JM109/pWM5Strain JM109/pWM5

Strain JM109/pWM5

Designation	Source	Preference
JM109/pWM5	Wanner B.L.	1

10 Mutations:

Mutation	Location	Certainty	Display
DE(gpt-lac)0	5.51	1	Δ(gpt-lac)0
glnX44(AS)	14.99	1	glnX44(AS)
LAM-	17.40	1	λ^-
rfbC1	45.43	0	rfbC1
gyrA96(NalR)	50.33	1	gyrA96(NalR)
recA1	60.80	1	recA1
endA1	66.57	1	endA1
spoT1	82.34	0	spoT1
thiE1	90.34	1	thiE1
hsdR17	98.74	1	hsdR17

Comments:
Episome/Plasmid: pWM5
Plasmid Markers/Mutations:
aadA(strR,SpcR)
bla(ApR)
MCS-2
uidA(p-)
Episome/Plasmid: F128-x
Plasmid Markers/Mutations:
lacIp-4000(lacIQ)
lacZ58(del)(M15)
traD36
proA
proB
References:
- Yanisch-Perron, C, J Vieira, J Messing 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33(1):103-19.
- Metcalf, W.W., B.L. Wanner 1993. Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129:17-25
Printable Strain Report