Mutation - fhuA766(del)::kan

Mutation - fhuA766(del)::kanMutation fhuA766(del)::kan

Mutation fhuA766(del)::kan

Name: fhuA766(del)::kan
Type: Insertion
Mutation of Gene: fhuA
Approx. Map Location: 3.61
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were ATCATTCTCGTTTACGTTATCATTCACTTTACATCAGAGATATACCAATGATTCCGGGGATCCGTCGACC and GCACGGAAATCCGTGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11

Property	Symbol
deletion	del
kanamycin resistant

3 Strains Carrying This Mutation

Name	Mutations	Genotype
JW0146-2	7	F-, Δ(araD-araB)567, ΔfhuA766::kan, ΔlacZ4787(::rrnB-3), λ^-, rph-1, Δ(rhaD-rhaB)568, hsdR514
WTZ17016	8	F-, Δ(araD-araB)567, ΔfhuA766::kan, ΔlacZ4787(::rrnB-3), λ^-, ΔiscR877::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR514
WTZ18003	14	F-, Δ(araA-leu)7697, [araD139]_B/r, ΔfhuA766::kan, Δ(codB-lacI)3, galK16, galE15(GalS), λ^-, e14-, mcrA0, relA1, rpsL150(strR), spoT1, mcrB1, hsdR2