Mutation mdtG723(del)::kan
- Name: mdtG723(del)::kan
- Type: Insertion
- Mutation of Gene: mdtG
- Approx. Map Location: 24.00
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were CCGCTTTTGCAAGTATGAAAGGCGAAAAATCAGTTCGATACCTGGGGTATATTCCGGGGATCCGTCGACC and TTTTCTTCTTATGTTCACCGCGCCTGGCGCACCAACAGCGGATTGCTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1040-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔmdtG723::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514