Mutation thrL721(del)::kan
- Name: thrL721(del)::kan
- Type: Insertion
- Mutation of Gene: thrL
- Approx. Map Location: 0.00
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGATTCCGGGGATCCGTCGACC and CGGGCTTTTTTCTGTGTTTCCTGTACGCGTCAGCCCGCACCGTTACCTGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4367-2 7 F-, ΔthrL721::kan, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514