Mutation - lacZ8(Am)

Mutation - lacZ8(Am)Mutation lacZ8(Am)

Mutation lacZ8(Am)

Name: lacZ8(Am)
Type: Base Pair Substitution
Mutation of Gene: lacZ
Approx. Map Location: 7.81
Synonyms
Designation Where Used
lac ull8am Normanly et al., 1990
Properties
Property Symbol
amber(UAG) mutation Am
Comments
- From Miller's CSH manual: carries amber mutation at position 17 of lacZ (Kleina et al. 1990)
References
- Normanly, J., L.G. Kleina, J.M. Masson, J. Abelson 1990. Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity. J.Mol.Biol. 213:719-726

Designation	Where Used
lac ull8am	Normanly et al., 1990

Property	Symbol
amber(UAG) mutation	Am

2 Strains Carrying This Mutation

Name	Mutations	Genotype
N2020	7	F-, lacZ8(Am), trpA36, glyA34, argA52, rpsL999(strR), rnpA49(ts), ilvG866(Act)
PN104	15	Hfr(PO2A), garB10, fhuA22, lacZ8(Am), phoA4(Am), λ^-, ompF627(T2R), fadL701(T2R), relA1, pitA10, dinD1::Mud(Ap,lac), spoT1, priA1::KanR, rrnB-2, mcrB1, creC510