Mutation - opgB202::Tn10

Mutation - opgB202::Tn10Mutation opgB202::Tn10

Mutation opgB202::Tn10

Name: opgB202::Tn10
Type: Tn
Mutation of Gene: opgB
Approx. Map Location: 99.04
Synonyms
Designation Where Used
mdoB202::Tn10
zii-202
zij-202
Properties
Property Symbol
tetracycline resistant
Comments
- cotransduces 34% with serB and 13% with thr and ca. 50% with hsd. Shown by PCR analysis (Nichols et al.) to be within opgB at nucleotide 4,595,002 (99.0 min.).
References
- Wertman, K.F., A.R. Wyman, D. Botstein 1986. Host/vector interactions which affect the viability of recombinant phage lambda clones. Gene 49:253-262
- Nichols, BP, O Shafiq, V Meiners 1998. Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180(23):6408-11.

Property	Symbol
tetracycline resistant

10 Strains Carrying This Mutation

Name	Mutations	Genotype
CAG18430	3	F-, λ^-, rph-1, opgB202::Tn10
DB1316	5	F-, λ^-, recD1014(Nuc-), mcrB1, hsdR2, opgB202::Tn10
DB1318	5	F-, λ^-, recA938::Tn9-200, recD1014(Nuc-), hsdR2, opgB202::Tn10
DB1317	6	F-, λ^-, tyrT58(AS), trp-89::Tn5, recD1014(Nuc-), hsdR2, opgB202::Tn10
SK596	6	Hfr(PO1), λ^-, e14-, relA1, spoT1, thiE1, opgB202::Tn10
DB1319	7	F-, λ^-, tyrT58(AS), trp-89::Tn5, recA938::Tn9-200, recD1014(Nuc-), hsdR2, opgB202::Tn10
MOB145	7	Hfr(PO1), gal-55, LAM?, relA1, spoT1, thiE1, hsdR4, opgB202::Tn10
SK472	7	Hfr(PO1), λ^-, e14-, relA1, spoT1, thiE1, opgB202::Tn10, serB22
W3110trpC720(del)	7	F-, λ^-, ΔtrpC720, IN(rrnD-rrnE)1, rph-1, tnaA5, hsdR2, opgB202::Tn10
LCK8	8	F-, lacY1 or Δ(cod-lacI)6, glnX44(AS), galK2(Oc), galT22, λ^-, metB1, hsdR2, opgB202::Tn10