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Site dnaX
- Name: dnaX
- Type: Gene
- Synonyms
b0470 MG1655 locus tag dnaZ mutH - Mnemonic: DNA
- Left End Point: 10.59
- Right End Point: 10.63
- Products:
DNA elongation factor III DNA polymerase III tau and gamma subunits gamma complex clamp loader - Direction: >
- Properties:
Property Comment DNA biosynthesis NULL - Priority: 1
- 4 Alleles of This Gene
dnaX0 dnaX2016 dnaX36(ts) dnaX797-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank M38777 EcoGene.org EG10245 EcoliWiki dnaX - Comment:
- Half the ribosomes frameshift internally and terminate to produce a short product. Tau is full-length product, gamma the short, frame-shifted product. Holds together 2 catalytic cores of holoenzyme.
- References:
- Hubscher, U., A. Kornberg 1979. The delta subunit of Escherichia coli DNA polymerase III holoenzyme is the dnaX gene product. Proc.Natl.Acad.Sci.USA 76:6284-6288
- Rowen, L., J. Kobori, S. Scherer 1982. Cloning of bacterial DNA replication genes in bacteriophage lambda. Mol.Gen.Genet. 187:501-509
- Mullin, D.A., C.L. Woldringh, J.M. Henson, J.R. Walker 1983. Cloning of the Escherichia coli dnaZX region and identification of its product. Mol.Gen.Genet. 192:73-79
- Kodaira, M., S.B. Biswas, A. Kornberg 1983. The dnaX gene encodes the DNA polymerase III holoenzyme T subunit, precursor of the y subunit, the dnaZ gene product. Mol.Gen.Genet. 192:80-86
- Blinkowa, A., W.G. Haldenwang, J.A. Ramsey, J.M. Henson, D.A. Mullin, J.R. Walker 1983. Physiological properties of cold-sensitive suppressor mutations of a temperature-sensitive dnaZ mutant of Escherichia coli. J.Bacteriol. 153:66-75
- Yin, K.-C., A. Blinkowa, J.R. Walker 1986. Nucleotide sequence of the Escherichia coli replication gene dnaZX. Nucleic Acids Res. 14:6541-6550
- Flower, A.M., C.S. McHenry 1986. The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame. Nucleic Acids Res. 14:8091-8101
- Lee, S., J.R. Walker 1987. Escherichia coli DnaX product, the tau subunit of DNA polymerase III, is a multifunctional protein with single-stranded DNA-dependent ATPase activity. Proc.Natl.Acad.Sci.USA 84:2713-2717
- Lee, S.H., P. Kanda, R.C. Kennedy, J.R. Walker 1987. Relation of the Escherichia coli dnaX gene to its two products, the tau and gamma subunits of DNA polymerase III holoenzyme. Nucleic Acids Res. 15:7663-7675
- Hawker, J.R., C.S. McHenry 1987. Monoclonal antibodies specific for the tau subunit of the DNA polymerase III holoenzyme of Escherichia coli. Use to demonstrate that tau is the product of the dnaZX gene and that both it and gamma, the dnaZ gene product
- Maki, S., A. Kornberg 1988. DNA polymerase III holoenzyme of Escherichia coli. I. Purification and distinctive functions of subunits tau and gamma, the dnaZX gene products. J.Biol.Chem. 263:6547-6554
- Tsuchihashi, Z., A. Kornberg 1989. ATP interactions of the tau and gamma subunits of DNA polymerase III holoenzyme of Escherichia coli. J.Biol.Chem. 264:17790-17795
- Tsuchihashi, Z., A. Kornberg 1990. Translational frameshifting generates the gamma subunit of DNA polymerase III holoenzyme. Proc.Natl.Acad.Sci.USA 87:2516-2520
- Flower, A.M., C.S. McHenry 1990. The gamma subunit of DNA polymerase III holoenzyme of Escherichia coli is produced by ribosomal frameshifting. Proc.Natl.Acad.Sci.USA 87:3713-3717
- Blinkowa, A.L., J.R. Walker 1990. Programmed ribosomal frameshifting generates the Escherichia coli DNA polymerase III gamma subunit from within the tau subunit reading frame. Nucleic Acids Res. 18:1725-1729
- Flower, A.M., C.S. McHenry 1991. Transcriptional organization of the Escherichia coli dnaX gene. J.Mol.Biol. 220:649-658
- Chen, K.S., P. Saxena, J.R. Walker 1993. Expression of the Escherichia coli dnaX gene. J.Bacteriol. 175:6663-6670
- Larsen, B., N.M. Wills, R.F. Gesteland, J.F. Atkins 1994. rRNA-mRNA base pairing stimulates a programmed -1 ribosomal frameshift. J.Bacteriol. 176:6842-6851
- Kelman, Z., M.A. O'Donnell 1995. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine. Annu.Rev.Biochem. 64:171-200
- Dallmann, H.G., R.L. Thimmig, C.S. McHenry 1995. DnaX complex of Escherichia coli DNA polymerase III holoenzyme. Central role of tau in initiation complex assembly and in determining the functional asymmetry of holoenzyme. J.Biol.Chem. 270:29555-29562
- Dallmann, H.G., C.S. McHenry 1995. DnaX complex of Escherichia coli DNA polymerase III holoenzyme. Physical characterization of the DnaX subunits and complexes. J.Biol.Chem. 270:29563-29569
- Kim, S., H.G. Dallmann, C.S. McHenry, K.J. Marians 1996. tau couples the leading- and lagging-strand polymerases at the Escherichia coli DNA replication fork. J.Biol.Chem. 271:21406-21412
- Kim, S., H.G. Dallmann, C.S. McHenry, K.J. Marians 1996. Tau protects beta in the leading-strand polymerase complex at the replication fork. J.Biol.Chem. 271:4315-4318
- Larsen, B., R.F. Gesteland, J.F. Atkins 1997. Structural probing and mutagenic analysis of the stem-loop required for Escherichia coli dnaX ribosomal frameshifting: programmed efficiency of 50%. J.Mol.Biol. 271:47-60
- Levine, C., K.J. Marians 1998. Identification of dnaX as a high-copy suppressor of the conditional lethal and partition phenotypes of the parE10 allele. J.Bacteriol. 180:1232-1240