Strain - JP5127

Strain - JP5127Strain JP5127

Strain JP5127

Designation	Source	Preference
JP5127	Russell R.R.B.	1

15 Mutations:

Mutation	Location	Certainty	Display
tsx-358	9.28	1	tsx-358
glnX44(AS)	14.99	0	glnX44(AS)
galE45(GalS)	17.03	1	galE45(GalS)
trp-356	28.00	1	trp-356
hisG4(Oc)	45.01	1	hisG4(Oc)
fucP-3	63.16	1	fucP-3
rpsL704(strR)	74.84	1	rpsL704(strR)
xylA5	80.34	1	xylA5
mtl-1	81.26	1	mtl-1
ilvC7	85.26	1	ilvC7
argE3(Oc)	89.48	1	argE3(Oc)
thiE1	90.34	1	thiE1
LAMR	1000.00	1	λ^R
tfr-3	1000.00	0	tfr-3
mal358	1000.00	1	mal358

Comments:
- 1. R.R.B. Russell and A. Egan suggest these procedures for use of JP5127 for the transduction of rel:
- 2. The concentration of propanediol in minimal plates must be 0.2% at least. Transduction to growth on propanediol may require 5 days' incubation at 37 degrees C.
- 3. In some genetic backgrounds, there is no apparent cotransduction of fuc-3 with argA or rel.
- 4. It is suggested that as much as 0.1 ml of the undiluted transduction mixture be plated (as well as normal dilutions) as the frequency of transductions may be low.
References:
- Russell, RR 1973. Close linkage of prd and rel genes in Escherichia coli K-12. Mol. Gen. Genet. 124(4):369-70.
Printable Strain Report