Strain - EB34

Strain - EB34Strain EB34

Strain EB34

CGSC#: 5908
Strain Designation
Designation Source Preference
EB34 Birge E.A. 1
Sex(Hfr,F+,F-,or F'): Hfr
Hfr Point of Origin#: 12
PO Map Position: 68.80
Direction of Marker Transfer: Clockwise

11 Mutations:

Mutation	Location	Certainty	Display
DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
lacY1 or lacZ4	8.00	1	lacY1 or lacZ4
glnX44(AS)	14.99	0	glnX44(AS)
galK2(Oc)	16.99	1	galK2(Oc)
LAM-	17.40	1	λ^-
rpsD2001	74.12	1	rpsD2001
rpsE2126(SpcR)	74.20	1	rpsE2126(SpcR)
rpsL252(strR)	74.84	1	rpsL252(strR)
xylA5	80.34	1	xylA5
mtl-1	81.26	1	mtl-1
thiE1	90.34	1	thiE1

Comments:
- This strain is streptomycin and spectinomycin-sensitive, presumably due to suppression of the rpsE126(SpcR) locus by rpsD2001, as well as suppression of strep-dependence of the parent strain. (See Ref.below.)
References:
- Birge, EA, CG Kurland 1970. Reversion of a streptomycin-dependent strain of Escherichia coli. Mol. Gen. Genet. 109(4):356-69.
Printable Strain Report