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Strain BW19094/pWM77

• CGSC#: 8217
• Strain Designation

  Designation	Source	Preference
  BW19094/pWM77	Wanner B.L.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 4 Mutations:

  Mutation	Location	Certainty	Display
  DE(codB-lacI)3	7.60	1	Δ(codB-lacI)3
  phoA532(del)	8.64	1	ΔphoA532
  uidA3(del)::pir+	36.48	1	ΔuidA3::pir+
  DE(phnP-phnD)3330( phnC?)	92.93	1	Δ(phnP-phnD)3330( phnC?)

  
  
• Comments:
• Episome/Plasmid: pWM77

  Plasmid Markers/Mutations:
  bla(ApR)
  lacZ alpha
  MCS(KS)
  ori f1,-
  oriR101
  oriT RP4

• References:
  • Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
  
  

  Printable Strain Report