E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

• CGSC Home
• Scope of the Collection
• The Database
• Privacy Policy
• Acknowledgment of Support

Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

How to Request Strains or Information

• Contact Information
• Charges
• Reviving Strains
• Strains Not Found?

Other CGSC Information

• FAQ on Procedures
• Current Working Map
• 1998 MMBR Map
• 1998 MMBR Gene List
• Map Diagrams (PDFs)

Other Links

• E. coli Links
• Other Stock Collections
• Other Bio Links
• Yale Web

Funded by a grant from the National Science Foundation

Strain KL2734

• CGSC#: 8108
• Strain Designation

  Designation	Source	Preference
  KL2734	Murgola E.J.	1

  
  
• Sex(Hfr,F+,F-,or F'): F'
• 5 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  DE(tonB-trp)	28.00	1	Δ(tonB-trp)
  IN(rrnD-rrnE)1	73.74	1	IN(rrnD-rrnE)1
  rph-1	82.20	1	rph-1
  glyV55	94.63	1	glyV55

  
  
• Comments:
  • The episome is a derivative of the Fredericq F-prime and contains genes for colicins V and B and the tonB-cysB region of the chromosome that includes the trp operon, with a suppressible trpA mutation.
• Episome/Plasmid: F596-2

  Plasmid Markers/Mutations:
  colB+
  colI+
  colV+
  trpA1017(UGA,UGA15)
  tonB
  trp

• References:
  • Arkov, A.L., A. Mankin, E.J. Murgola 1998. An rRNA fragment and its antisense can alter decoding of genetic information. J.Bacteriol. 180:2744-2748
  • Chernyaeva, N.S., E.J. Murgola, A.S. Mankin 1999. Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA. J.Bacteriol. 181:5257-5262
  
  

  Printable Strain Report